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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
APC Anti-Human CD117/c-Kit Antibody流式抗体[104D2]_货号:E-AB-F1150E
- 抗体英文名:
APC Anti-Human CD117/c-Kit Antibody[104D2]_货号:E-AB-F1150E
- 靶点:
CD117
- 浓度:
5 μL/Test
- 应用范围:
FCM
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
12个月
- 级别:
科研
- 库存:
99
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 标记物:
APC
- 克隆性:
Monoclonal
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 形态:
液体
- 亚型:
Mouse IgG1, κ
- 规格:
100Tests/20Tests/100Tests×2
| 规格: | 100Tests | 产品价格: | ¥2880.0 |
|---|---|---|---|
| 规格: | 20Tests | 产品价格: | ¥1080.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥4440.0 |
货号:E-AB-F1150E
| Background |
CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.
|
| Alternate Names |
Mast/stem cell growth factor receptor Kit;Kit;SCFR;Proto-oncogene c-Kit;Tyrosine-protein kinase Kit;CD117;
|
| Swissprot |
P10721
|
| Gene ID |
3815
|
| Clone No |
104D2
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
APC Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
APC
|
| Conjugation Information |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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中。5. 用无菌 PBS 将上述锥形试管填满(至 50 mL)。介绍样品制备。6. 洗涤。在室温、低速制动条件下,以 300 X g 离心分离试管 10 分钟。7. 裂解红细胞(BD 产品目录号:555899,10 x 溶液。在蒸馏水中使用 1 x 溶液。室温孵育 15 分钟。)8. 将 1x106 PBMC 重悬于 20 μL 染色缓冲液中(PBS 含有 5% 正常人血清)。9. 加入抗体:CD117-BV421 (c-kit)、CD7-BV510、CD57- BV605、CD8-BV650
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灌洗液(BALF)免疫细胞群体的剧烈重塑。在LPS刺激24小时后,肺部微环境发生了从“稳态维持”向“急性炎症爆发”的根本性转变。中性粒细胞取代了原本的常驻细胞,成为BALF中的绝对优势群体,而肺泡巨噬细胞的比例则出现了断崖式下跌。 Elabscience®相关产品推荐 货号 产品名称 AN00871E APC 标记抗小鼠MERTK抗体[2B10C42] E-AB-F09832E APC 标记大鼠IgG2a,κ同型对照抗体[2A3] E-AB-F0990Q2 Elab Bright™
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