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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
PE/Cyanine7 Anti-Human CD45 Antibody流式抗体[HI30]_货号:E-AB-F1137H
- 抗体英文名:
PE/Cyanine7 Anti-Human CD45 Antibody[HI30]_货号:E-AB-F1137H
- 靶点:
CD45
- 浓度:
5 μL/Test
- 应用范围:
FCM
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
12个月
- 级别:
科研
- 库存:
99
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 标记物:
APC
- 克隆性:
Monoclonal
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 形态:
液体
- 亚型:
Mouse IgG1, κ
- 规格:
100Tests/20Tests/100Tests×2
| 规格: | 100Tests | 产品价格: | ¥2736.0 |
|---|---|---|---|
| 规格: | 20Tests | 产品价格: | ¥1200.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥3312.0 |
货号:E-AB-F1137H
| Background |
CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
|
| Alternate Names |
Receptor-type tyrosine-protein phosphatase C;Ptprc;L-CA;Ly-5;T200;CD45;
|
| Swissprot |
P08575
|
| Gene ID |
5788
|
| Clone No |
HI30
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
PE/Cyanine7 Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
PE/Cyanine7
|
| Conjugation Information |
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
IF:1.3
Journal:MEDICINE
DOI:10.1097/MD.0000000000039350
Human embryonic stem cells (hESCs) and patient-specific human induced pluripotent stem cells (hiPSCs) are valuable reagents for studying the earliest stages of hematopoietic genesis and for modeling the developmental basis of hematologic
69、CD38 等活化指标,及 IFN-γ、IL-4、IL17A 等细胞因子,则需要设置同型对照,实现阴阳性细胞群的良好区分。 CD4 CD25 (2)在某些特殊情况下,如单核细胞被 PE/Cyanine 7、APC/Cyanine 7 等串联染料标记的抗体染色时,使用同型对照抗体是十分有必要的。单核细胞一般倾向于与 Cyanine7 等染料非特异性结合,使用同型对照抗体有助于获得理想结果。 (3)不清楚抗原表达情况的时候,一定要使用同型对照来界定阴性细胞群。 (4)胞内染色时
、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、 CD45-AF700、CD38-APC-AF750(每次测试 5μL),以及 CD16-FITC(每次测试 20 μL),并在冰上孵育 30 分钟。10. 在 4℃ 条件下,300 x G 离心细胞 5 分钟。11. 吸取上清液。12. 重悬到 500 μL 染色缓冲液中。13. 通过 CytoFLEX 获取结果。通过 CytoFLEX 进行数据采集:1. 创建新补偿值。2. 如适用
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