- ￥760 - 4760
- Elabscience
- E-AB-F11330
- 武汉
- 2026年03月25日
- FCM;Block;FA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
AF/LE Purified Anti-Human CD274/PD-L1 Antibody流式抗体[29E.2A3]_货号:E-AB-F11330
- 抗体英文名：
AF/LE Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3]_货号:E-AB-F11330
- 靶点：
PD-L1
- 浓度：
5 μL/Test
- 应用范围：
FCM;Block;FA
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
12个月
- 级别：
科研
- 库存：
99
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 标记物：
PE/Cyanine 7
- 克隆性：
Monoclonal
- 保存条件：
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
- 形态：
液体
- 亚型：
Mouse IgG2b, κ
- 规格：
500μg/50μg/1mg
| 规格： | 500μg | 产品价格： | ￥3160.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥760.0 |
| 规格： | 1mg | 产品价格： | ￥4760.0 |
货号：E-AB-F11330
| Background |
CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.
|
| Alternate Names |
B7-H1; PD-L1;Programmed cell death ligand 1;B7 homolog 1;B7-H; B7H1; PDL1; PDCD1L1; PDCD1LG1;
|
| Swissprot |
Q9NZQ7
|
| Gene ID |
29126
|
| Clone No |
29E.2A3
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM;Block;FA
|
| Isotype |
Mouse IgG2b, κ
|
| Isotype Control |
AF/LE Purified Mouse IgG2b, κ Isotype Control[MPC-11]
|
| Conjugation |
None (Purified antibody-Azide Free/Low endotoxin)
|
| Storage Buffer |
0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Upregulation of Immune checkpoint PD-L1 in Colon cancer cell lines and activation of T cells by Leuconostoc mesenteroides
IF:4
Journal:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
DOI:10.1007/s11274-024-04018-7
Phycoerythrin conjugation protocol
1 ml purified antibody at approx 2.5 mg/ml in PBS. Antibody must be fairly pure - i.e. purify on protein A or G column before conjugating. Add 20 µl SMCC, 1.7 mg/ml in DMSO. Incubate for 1 hour. Take product from step (2) and add
domesticus x Mus spretus] F1 DNA). In the analysis of the antibody bound (B) and unbound (U) fractions (right panel), the bands representing the maternal and paternal alleles are indicated.MATERIALS Reagents100-bp DNA step ladder (for duplex PCR
). The final (F1 + F2 + F3) PCR product was digested with SfI I and cloned in the pComb3X vector29 . The resulting pComb3X-3ZF library vector was used to construct the 6ZF library as follows. First, 10 g of pComb3X-3ZF library vector was digested with Age
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