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- Elabscience
- E-AB-F1104A
- 湖北武汉
- 2025年07月15日
- FCM
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Purified Anti-Mouse CD8a Antibody流式抗体[53-6.7]_货号:E-AB-F1104A
- 抗体英文名：
Purified Anti-Mouse CD8a Antibody[53-6.7]_货号:E-AB-F1104A
- 应用范围：
FCM
- 宿主：
Rat
- 适应物种：
Mouse
- 保质期：
12个月
- 目录编号：
E-AB-F1104A
- 级别：
科研
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
This product can be stored at 2-8°C for 12 months. Do not freeze.
- 形态：
液体
- 亚型：
Rat IgG2a, κ
- 规格：
100μg/25μg
| 规格： | 100μg | 产品价格： | ￥392.0 |
|---|---|---|---|
| 规格： | 25μg | 产品价格： | ￥152.0 |
货号：E-AB-F1104A
| Background |
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
|
| Alternate Names |
T-cell surface glycoprotein CD8 alpha chain;CD8A;T-lymphocyte differentiation antigen T8/Leu-2;MAL;
|
| Swissprot |
P01731
|
| Gene ID |
12525
|
| Clone No |
53-6.7
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2a, κ
|
| Isotype Control |
Purified Rat IgG2a, κ Isotype Control[2A3]
|
| Conjugation |
Unconjugated
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献详情查看>>>>
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
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