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- 文献和实验
- 技术资料
- 亚型:
Rat IgG2b, κ
- 形态:
液体
- 保存条件:
This product can be stored at 2-8°C for 12 months. Do not freeze.
- 克隆性:
Monoclonal
- 标记物:
None (Purified antibody-Azide Free/Low endotoxin)
- 适应物种:
Mouse
- 保质期:
12个月
- 目录编号:
E-AB-F10940
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Rat
- 应用范围:
FCM;Depletion;Apoptosis
- 抗体英文名:
AF/LE Purified Anti-Mouse CD90.2/Thy1.2 Antibody[30H12]_货号:E-AB-F10940
- 抗体名:
AF/LE Purified Anti-Mouse CD90.2/Thy1.2 Antibody流式抗体[30H12]_货号:E-AB-F10940
- 规格:
500μg/50μg/1mg
| 规格: | 500μg | 产品价格: | ¥3160.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥760.0 |
| 规格: | 1mg | 产品价格: | ¥4760.0 |

AF/LE Purified Anti-Mouse CD90.2/Thy1.2 抗体流式抗体[30H12]产品名称:AF/LE Purified Anti-Mouse CD90.2/Thy1.2 Antibody[30H12], 货号:E-AB-F10940
| 产品描述: | CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes. |
| Alternate Names | Thy-1.2 membrane glycoprotein;Thy1.2;Thy-1.2 antigen;CD90.2;Thy-1.2; |
| Gene ID | 21838 |
| Clone No | 30H12 |
| Host | Rat |
| Reactivity | Mouse |
| Application | FCM;Depletion;Apoptosis |
| 同种型 | Rat IgG2b, κ |
| 同种型 Control | AF/LE Purified Rat IgG2b, κ 同种型 Control[LTF-2] |
| Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
| 储存方法 缓冲 | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
| 储存方法 | This product can be stored at 2-8°C for 12 months. Do not freeze. |
| Expiration date | 12个月 |
| Shipping | 冰袋 |

AF/LE Purified Anti-Mouse CD90.2/Thy1.2 抗体流式抗体[30H12]labscience®拥有先进的单抗,多抗和流式抗体开发技术平台,提供8500+高品质 抗体, 适用于胞内和胞外蛋白的鉴定、定量和定位研究。抗体已在多种应用中得到验证,包含 流式细胞术(Flow Cytometry)、 免疫印迹(WB)、 免疫组化(IHC/ICC)、 免疫(共)沉淀(IP/Co-IP)、 免疫荧光(IF) 等实验。抗体产品涉及多种生命科学研究领域,包括肿瘤、免疫、心血管、细胞生物学、神经科学、表观遗传学等。Elabscience®始终致力于提高抗体的质量和种类,以保障并加快您的研究进程
This product can be stored at 2-8°C for 12 months. Do not freeze.AF/LE Purified Anti-Mouse CD90.2/Thy1.2 抗体流式抗体[30H12]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验分泌。 随后进行表面染色:先以Fc阻断剂(2.4G2克隆,5 μg/mL)处理10分钟,再于4°C避光孵育15分钟,使用含1% BSA和0.1% NaN₃的DPBS缓冲液配制抗体,常用组合包括CD90.2、CD4、CD8、CD25、CD11b和CD45.2等标记。 固定步骤采用2%甲醛(FA,由含<3%甲醇的4% Roti-Histofix稀释),室温孵育40–60分钟。破膜与胞内染色使用0.1%皂苷缓冲液或1×Perm缓冲液,加入抗FoxP3、T-bet、RORγt、IL-17A或IFN-γ等抗体,4°C
分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
7-氨基放线菌su D(7-AAD)染色液 K115 DAPI染色液 K117 刺激阻断 细胞因子激活和蛋白阻断试剂盒 K083 固定/破膜 Foxp3/转录因子染色固定&破膜试剂盒 K081 胞内因子固定破膜剂 (即用型) K082R FcR封闭 Purified Anti-Mouse CD16/32 Antibody FNab30106 Purified
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