- ￥5600
- GeneTex
- 美国
- GTX10695
- 2025年07月12日
- WB, IHC-P
- Rabbit
- Human, Mouse, Chicken
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human RSK1 that contains serine 221. The sequence is conserved in mouse and rat.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Chicken
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX10695
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
p90 RSK1 (phospho Ser221)
- 抗体英文名：
p90 RSK1 (phospho Ser221) antibody
- 抗体名：
p90 RSK1 (phospho Ser221) 抗体
- 规格：
50 μl
WB (peptide competition) analysis of untreated A431 (Lane 1) or PMA-stimulated A431 cells (Lane 2~5) using GTX10695 p90 RSK1 (phospho Ser221) antibody prior incubated with the non-phosphopeptide (Lane 3), a generic [pS] peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
IHC-P analysis of human brain tissue using GTX10695 p90 RSK1 (phospho Ser221) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retireval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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