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- Elabscience
- 湖北武汉
- E-AB-F1052D
- 2025年07月11日
- FCM
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Mouse IgG2b, κ
- 形态：
液体
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性：
Monoclonal
- 标记物：
PE
- 适应物种：
Human
- 保质期：
12个月
- 目录编号：
E-AB-F1052D
- 级别：
科研
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 宿主：
Mouse
- 应用范围：
FCM
- 抗体英文名：
PE Anti-Human CD45RA Antibody[HI100]_货号:E-AB-F1052D
- 抗体名：
PE Anti-Human CD45RA Antibody流式抗体[HI100]_货号:E-AB-F1052D
- 规格：
100Tests/20Tests/100Tests×2
| 规格： | 100Tests | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 20Tests | 产品价格： | ￥336.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥1656.0 |
货号：E-AB-F1052D
| Background |
CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.
|
| Alternate Names |
Receptor-type tyrosine-protein phosphatase C;Ptprc;L-CA;Ly-5;T200;CD45;
|
| Swissprot |
P08575
|
| Gene ID |
5788
|
| Clone No |
HI100
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2b, κ
|
| Isotype Control |
PE Mouse IgG2b, κ Isotype Control[MPC-11]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献详情查看>>>>
抗[E-AB-F0997A]进行封闭：往 100 μL（细胞数1×106）细胞悬液中加入 1 μg 纯抗，室温封闭 15 min，直接加入流式抗体进行后续实验。 巨噬细胞检测应尽量避免使用含花青素的流式抗体（如 PE-Cy7），会增加非特异性染色。若为非巨噬细胞检测，使用含花青素的流式抗体（如 PE-Cy7）时也需要封闭。
T-cell dependent antigen specific in situ staining (Pe)
HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’. Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
the same antigen is the antibody TG343 (Millipore, cat. no. MAB4346) PE rat anti‐mouse CD 90.2 (0.2 mg/ml; BD Pharmingen, cat. no. 553014)
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