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- Elabscience
- 湖北武汉
- E-AB-F1052D
- 2026年05月27日
- FCM
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Mouse IgG2b, κ
- 形态：
液体
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性：
Monoclonal
- 标记物：
PE
- 适应物种：
Human
- 保质期：
12个月
- 目录编号：
E-AB-F1052D
- 级别：
科研
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 宿主：
Mouse
- 应用范围：
FCM
- 抗体英文名：
PE Anti-Human CD45RA Antibody[HI100]_货号:E-AB-F1052D
- 抗体名：
PE Anti-Human CD45RA Antibody流式抗体[HI100]_货号:E-AB-F1052D
- 规格：
100Tests/20Tests/100Tests×2
| 规格： | 100Tests | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 20Tests | 产品价格： | ￥336.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥1656.0 |
货号：E-AB-F1052D
| Background |
CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.
|
| Alternate Names |
Receptor-type tyrosine-protein phosphatase C;Ptprc;L-CA;Ly-5;T200;CD45;
|
| Swissprot |
P08575
|
| Gene ID |
5788
|
| Clone No |
HI100
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2b, κ
|
| Isotype Control |
PE Mouse IgG2b, κ Isotype Control[MPC-11]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献详情查看>>>>
抗[E-AB-F0997A]进行封闭：往 100 μL（细胞数1×106）细胞悬液中加入 1 μg 纯抗，室温封闭 15 min，直接加入流式抗体进行后续实验。 巨噬细胞检测应尽量避免使用含花青素的流式抗体（如 PE-Cy7），会增加非特异性染色。若为非巨噬细胞检测，使用含花青素的流式抗体（如 PE-Cy7）时也需要封闭。
T-cell dependent antigen specific in situ staining (Pe)
HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’. Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide
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