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- 文献和实验
- 技术资料
- 亚型:
Mouse IgG2a, κ
- 形态:
液体
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性:
Monoclonal
- 标记物:
FITC
- 适应物种:
Human
- 保质期:
12个月
- 目录编号:
E-AB-F1038C
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Mouse
- 应用范围:
FCM
- 抗体英文名:
FITC Anti-Human CD44 Antibody[P2A1]_货号:E-AB-F1038C
- 抗体名:
FITC Anti-Human CD44 Antibody流式抗体[P2A1]_货号:E-AB-F1038C
- 规格:
100Tests/20Tests/100Tests×2
| 规格: | 100Tests | 产品价格: | ¥1740.0 |
|---|---|---|---|
| 规格: | 20Tests | 产品价格: | ¥480.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥2640.0 |
货号:E-AB-F1038C
| Background |
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from a low or mid level of intensity to high expression levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
|
| Alternate Names |
CD44 antigen;CD44;CDw44;Epican;Phagocytic glycoprotein 1;PGP-1;Phagocytic glycoprotein I;PGP-I;CD44;LHR; MDU2; MDU3; MIC4;
|
| Swissprot |
P16070
|
| Gene ID |
960
|
| Clone No |
P2A1
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
FITC Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
FITC
|
| Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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Elabscience | BV2细胞M1极化造模与流式检测,这份Protocol请收好
Buffer(E-CK-A107)或PBS缓冲液重悬细胞沉淀,调整细胞密度为1-10×106个/mL; (3)取100 μL细胞悬液,每组加入2 μL Purified Anti-Mouse CD16/32 Antibody(E-AB-F0997A),轻轻吹打混匀,室温避光孵育10 min封闭细胞表面的Fc受体; (4)加入各流式抗体(5 μL/test),轻轻吹打混匀,4℃避光孵育30 min; (5)孵育完成后,每管加入1 mL Cell Staining Buffer(E-CK-A107)轻轻
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Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
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