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- Elabscience
- 湖北武汉
- E-AB-F1026L
- 2025年07月13日
- FCM
- Syrian Hamster
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Syrian Hamster IgG
- 形态：
液体
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性：
Monoclonal
- 标记物：
Elab Fluor<sup>®</sup> 488
- 适应物种：
Mouse
- 保质期：
12个月
- 目录编号：
E-AB-F1026L
- 级别：
科研
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 宿主：
Syrian Hamster
- 应用范围：
FCM
- 抗体英文名：
Elab Fluor® 488 Anti-Mouse CD28 Antibody[37.51]_货号:E-AB-F1026L
- 抗体名：
Elab Fluor® 488 Anti-Mouse CD28 Antibody流式抗体[37.51]_货号:E-AB-F1026L
- 规格：
100Tests/50Tests/100Tests×2
| 规格： | 100Tests | 产品价格： | ￥1320.0 |
|---|---|---|---|
| 规格： | 50Tests | 产品价格： | ￥780.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥2040.0 |
货号：E-AB-F1026L
| Background |
CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.
|
| Alternate Names |
T-cell-specific surface glycoprotein CD28;Cd28;CD28;
|
| Swissprot |
P31041
|
| Gene ID |
12487
|
| Clone No |
37.51
|
| Host |
Syrian Hamster
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Syrian Hamster IgG
|
| Isotype Control |
Elab Fluor® 488 Syrian Hamster IgG Isotype Control[SHG-1]
|
| Conjugation |
Elab Fluor<sup><sup>®</sup></sup> 488
|
| Conjugation Information |
Elab Fluor® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验自2012年第一篇引用文献问世，至2023年8月，Elabscience已协助各地科研工作者发表超13,000篇SCI文献，包括行业顶/级期刊Nature Medicine, Nature, Cell, Signal Transduction and Targeted Therapy, Circulation, Molecular Cancer, Immunity, Advanced Materials等，总影响因子（IF）59472.59，其中IF≥30的文献超30篇，最高影响因子为66.849，发文单位包含北京大学、中国科学技术大学、浙江大学、斯坦福大学、耶鲁大学、柏林夏里特大学等国内外各大高校。
每年引用文献数量持续增长，保持逐年30%+高增长率，可以预见，Elabscience产品将帮助更多科研工作者发表高质量SCI论文。
文献详情查看>>>>
分钟收集细胞； 5. 将上一步离心得到的细胞沉淀，重悬至扩增培养基中，并进行计数。 1.3 细胞刺激（次日） 扩增培养基成分：RPMI1640 + 10% 血清 + 5ug/ml anti-mouse CD28（克隆号：37.51） + 双抗 1. 将细胞浓度调整至 1-2×10^6/ml（本次实验为 2×10^6/ml，该浓度偏大）； 2. 从 4°C 冰箱取出前一天包被的板子，弃掉包被液，无菌PBS洗涤3遍； 3. 每孔加入 500μl 细胞悬液，放置于 37°C 含 5% CO2 细胞
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
volume of Dynabeads taken from the vial (step 2). 2. Activation of Mouse T Cells 1) Start with 8 × 104 purified T cells in 100-200 µl medium in a 96-well tissue culture plate. 2) Add 2 µl Dynabeads Mouse T-Activator CD3/CD28
using Dynabeads® CD3⁄CD28 CTS™. Following separation, the CD3 T cells are cultured in the presence of the beads. By combining anti-CD3 and anti-CD28 antibodies on Dynabeads®, the beads will provide both the primary and co-stimulatory signals
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