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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntaxin 1a. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX106365
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF
- 浓度:
9.3 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Syntaxin 1a
- 抗体英文名:
Syntaxin 1a antibody [N1], N-term
- 抗体名:
Syntaxin 1a 抗体 [N1], N-term
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Syntaxin 1a antibody [N1], N-term detects Syntaxin 1a protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% PFA at RT for 15 min.
Green: Syntaxin 1a protein stained by Syntaxin 1a antibody [N1], N-term (GTX106365) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX106365 diluted at 1:1000
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Methods for the Study of Intermolecular and Intramolecular Interactions Regulating CFTR Function
family of proteins have been implicated in membrane fusion (3 ,5 ). In the synapse, N-type calcium channels bind to syntaxin 1A (8 ,8 ), which negatively modulates the gating of these channels (9 ,11 ). The carboxy terminal tail interacts with PDZ
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