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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human serum albumin
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX10244
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ELISA, Sandwich ELISA
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Does not react with bovine serum albumin, egg white albumin or human alpha fetoprotein.
- 抗体英文名:
Albumin antibody [6B11]
- 抗体名:
Albumin 抗体 [6B11]
- 规格:
100 μg
Albumin staining by Human Albumin antibodies in Western blotting after electrophoretic separation of human plasma proteins under reducing conditions. Lane 1: Human Albumin antibody [6B11] (GTX10244); Lane 2: Human Albumin antibody [15C7] (GTX10241)
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文献和实验environment with little denaturation. Purified antibodies: Once purified, if the antibody is at less than 0.1 mg/ml add a small amount of detergent or protein (0.1% TWEEN-20 or 1% bovine serum albumin) and some protease inhibitors to the solution
volumes running buffer (the serum albumin should stick to the column and the Ig should flow through).(6) On ice slowly add saturated ammonium sulfate to 45% (550 ml sample 450 ml saturated ammonium sulfate). Tilt overnight at 4°C.(7) Pellet by spinning
Antibody Purification This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography. Solutions Affigel Blue Prewash 0.1 M acetic acid 5.7 ml glacial acetic acid
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