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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human beta Tubulin 4. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX102095
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF
- 浓度:
0.41 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
beta Tubulin 4
- 抗体英文名:
beta Tubulin 4 antibody
- 抗体名:
beta Tubulin 4 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Sample(30 μg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX102095 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX102095 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed U2OS, using beta Tubulin 4(GTX102095) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
beta Tubulin 4 antibody detects beta Tubulin 4 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% PFA at RT for 15 min.
Green: beta Tubulin 4 stained by beta Tubulin 4 antibody (GTX102095) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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文献和实验Huang HS et al., Carcinogenesis 2015 (PMID:26363031)
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
) antibodies and anti-beta 2 glycoprotein I (β2-GPI) antibodies. Other autoantibodies, such as those directed against anionic phospholipids, can also be assayed; however they are not of clinical significance. Participation in a quality assurance
Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
。 如果beta-catenin抗体不好使,或者EGFP太大影响定位的话,还可以构建Flag标签类载体,转染细胞,加入药物,用抗Flag抗体免疫荧光检测,荧光显微镜下观察就好了。 wangjh28 但按照事先的推测,处理了以后会下调该基因的表达 我觉得科学不能有事先的推测的结果,这样容易受思维定势的影响,而忽略了新发现 dongreyuan 我打算用SAB的β-catenin(Ab-37)Antibody,说明
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