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- 详细信息
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human RAP1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX101983
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
RAP1
- 抗体英文名:
RAP1 antibody [N2C3]
- 抗体名:
RAP1 抗体 [N2C3]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP1 antibody [N2C3] (GTX101983) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using RAP1(GTX101983) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
RAP1 antibody [N2C3] detects RAP1 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% PFA at RT for 15 min.
Green: RAP1 protein stained by RAP1 antibody [N2C3] (GTX101983) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101983 diluted at 1:1000
RAP1 antibody immunoprecipitates RAP1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of RAP1 by 3 μg of RAP1 antibody (GTX101983) 10% SDS-PAGE The immunoprecipitated RAP1 protein was detected by RAP1 antibody (GTX101983) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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