- ￥1700 - 4000
- GeneTex
- 美国
- GTX101908
- 2025年07月13日
- WB, ICC/IF, IHC-P, IHC-Fr
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the N-terminus region of human VGAT. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX101908
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr
- 浓度：
1.08 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
VGAT
- 抗体英文名：
VGAT antibody [N1N2], N-term
- 抗体名：
VGAT 抗体 [N1N2], N-term
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
VGAT antibody [N1N2], N-term detects VGAT protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% PFA at RT for 15 min.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
VGAT antibody [N1N2], N-term detects VGAT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: VGAT stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
VGAT antibody [N1N2], N-term detects VGAT protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
VGAT antibody [N1N2], N-term detects VGAT protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron and Glia cells were fixed in 4% PFA at RT for 15 min. Green: VGAT stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250. Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using VGAT(GTX101908) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Boiled and unboiled mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Schmeichel AM et al., Sleep 2021 (PMID:33755181)
Sato M et al., Neuropathol Appl Neurobiol 2020 (PMID:32722888)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain
Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust
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