- ￥1700 - 4000
- GeneTex
- 美国
- GTX101435
- 2025年07月16日
- WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, FACS, IP, ChIP assay, IHC
- Rabbit
- Human, Mouse, Rat, Zebrafish, Rabbit, Cat, Dog
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Zebrafish, Rabbit, Cat, Dog
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX101435
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, FACS, IP, ChIP assay, IHC
- 浓度：
0.07 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
beta Catenin
- 抗体英文名：
beta Catenin antibody [N1N2-2], N-term
- 抗体名：
beta Catenin 抗体 [N1N2-2], N-term
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (9 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed A431, using beta Catenin(GTX101435) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Evdokia Bogdanova et al., Int J Mol Sci 2021 (PMID:33924991)
Chi-Cheng Lu et al., Exp Ther Med 2021 (PMID:34131445)
Hsuan-Fu Kuo et al., Biomedicines 2021 (PMID:34680582)
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，后来发现与我买的beta-catenin一抗（中杉金桥分装的santa cruz的产品）说明书上的结果一致。请问大家做beta-catenin免疫荧光用的是哪里的抗体啊，或者是我的方法有问题吗？我也用0.5%TritonX-100打孔了，仍不行。我在这里先谢谢各位了！ zhao22840718 还有一种方法不知有没有人试过，就是把beta-catenin构建到pEGFP-N3类载体中，表达融合蛋白，把载体转入细胞，然后加入你的药物。荧光显微镜下观察进核情况
医生和球 看的文献较少，自己知道的只有wnt、PTEN等，请问还有哪些通路与β-catenin有关，有无类似的综述？谢谢！ 狐狸renart 只知道wnt chuckdouble The canonical Wnt/beta-catenin signalling pathway. Barker N. Methods Mol Biol. 2008;468:5-15
v468.Chapter 8 采用 b-Catenin/TCF基本转录通讯结构对b-Catenin/TCF转录进行分析
., Morin, P.J., van Wichen, D., de Weger, R., Kinzler, K.W., Vogelstein, B., and Clevers, H. (1997). Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC –/– colon carcinoma. Science 275 , 1784–1787. 5. Veeman, M.T., Slusarski
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