- ￥1700 - 4000
- GeneTex
- 美国
- GTX101316
- 2025年07月13日
- WB, ICC/IF, IHC-P, IP, Immunosubtraction
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX101316
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP, Immunosubtraction
- 浓度：
0.08 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
The mouse reactivity of this antibody is based on mouse IHC-P testing and may be not suitable for mouse WB assay.
- 抗体英文名：
C3 / C3b antibody [C3], C-term
- 抗体名：
C3 / C3b 抗体 [C3], C-term
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term (GTX101316) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: C3 protein stained by C3 antibody [C3], C-term (GTX101316) diluted at 1:200.
Blue: Hoechst 33342 staining.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody (GTX101316) diluted at 1:1000.
C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C3 antibody [C3], C-term (GTX101316) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg of C3 antibody [C3], C-term (GTX101316).
Western blot analysis was performed using C3 antibody [C3], C-term (GTX101316).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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文献和实验Kuroda Y et al., J Pharm Biomed Anal 2013 (PMID:23298910)
Cao K et al., Alzheimers Res Ther 2019 (PMID:31010414)
Gu矇guinou N et al., Int J Mol Sci 2019 (PMID:30934839)
Fukuoka Y et al., J Immunol 2013 (PMID:23833239)
Arai S et al., Cell Rep 2013 (PMID:23562157)
补体成分 C3(C3a/C3b) 补体成分：C3 血清浓度(μg/ml)：550-1200 分子量(kDa)：195 亚单位(链)及分子量(kDa)：α∶110；β∶85 激活产物：C3a；C3b 生物学活性：C3a 过敏毒素、趋化作用等。 C3b 组成CP、AP中的C3、C5转化
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠补体C3b (C3b)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液、唾液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 C3b 单抗包被于酶标板上，标准品和样品中的 C3b与单抗结合，加入生物素化的抗小鼠 C3b
链在半胱氨酸和谷氨酸残基之间也有一个内硫酯键（Cys-S-CO-Glu）。此环状结构水解后，可形成一个转移性结合点，认为这是C3b由液相结合到固相上的结构条件，也是C3以缓慢的速率水解导致其构象改变出现能与B因子具有亲和力的“变构”C3b的分子基础。当C3转化酶从C3α链N端一个精氨酸-丝氨酸键（第77-78位）外将C3裂解后，可产生一个9kDa的小片段C3a（释放到液相中去），其余部分为C3b。同时，新生的C3bα`链内距N端5kDa的硫酯键断裂，在谷氨酰胺基上出现一个具有转酯作用的高度或多糖
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