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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the N-terminus region of human Filamin B. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX101206
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
10 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Filamin B
- 抗体英文名:
Filamin B antibody [N1], N-term
- 抗体名:
Filamin B 抗体 [N1], N-term
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [N1], N-term (GTX101206) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Filamin B antibody detects Filamin B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody (GTX101206) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate)
A: U87-MG
5% SDS PAGE
GTX101206 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed HeLa, using Filamin B(GTX101206) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
Filamin B antibody [N1], N-term detects Filamin B protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell extract
5% SDS-PAGE
Filamin B antibody [N1], N-term (GTX101206) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Filamin B antibody [N1], N-term detects Filamin B protein at cytoplasm in human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Filamin B antibody [N1], N-term (GTX101206) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Surcel A et al., bioRxiv 2017 (Epub)
Yang CF et al., Hum Mutat 2017 (PMID:28145000)
Artym VV et al., J Cell Biol 2015 (PMID:25646088)
Yue Chen et al., Front Cardiovasc Med 2021 (PMID:34485398)
Li Y et al., Virol Sin 2018 (PMID:29594956)
Therapeutic strategies for cancer include chemotherapy, immunotherapy, and radiation. Such therapies result in significant short-term clinical responses; however, relapses and recurrences occur with no treatments. Targeted therapies using
b-Gal Staining of Murine Embryos
PO4.H2O, 77ml of 0.2M Na2HPO4 and add 100ml DDW. the pH will be approx 7.3 0.5M EGTA in 0.1M Phosphate buffer, pH increase to 7. FW 380.4, so 19.02g for making 100ml of 0.5M EGTA. Ethylene Glycol-bis (b-aminoethyl ether)N,N,N',N'-tetraacetic acid
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
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