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- 详细信息
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the C-terminus region of human NFkB p100. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX101150
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
GTX101150 specifically recognizes NFkB p100 isoform, but not the cleaved p52 isoform.
- 抗体英文名:
NFkB p100 antibody [C2C3], C-term
- 抗体名:
NFkB p100 抗体 [C2C3], C-term
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
NFkB p100 antibody [C2C3], C-term detects NFkB p100 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:1000.
NFkB p100/p52 antibody immunoprecipitates NFkB p100/p52 protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. 30 μg Jurkat whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p100/p52 protein by 2 μg of NFkB p100/p52 antibody (GTX101150) 5% SDS-PAGE The immunoprecipitated NFkB p100/p52 protein was detected by NFkB p100/p52 antibody (GTX101150) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Wild-type (WT) and NFkB p100 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
7.5% SDS PAGE
NFkB p100 antibody
GTX101150 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A:NIH-3T3 7.5% SDS PAGE
GTX101150 diluted at 1:1000
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![NFkB p100 antibody [C2C3], C-term](https://img1.dxycdn.com/2023/0711/255/2344116916935858761-14.jpg)



