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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the N-terminus region of human PPAR alpha. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Honeybee, Queen Bee, Plant
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX101098
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P, IP, ChIP assay
- 浓度:
0.39 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
PPAR alpha
- 抗体英文名:
PPAR alpha antibody
- 抗体名:
PPAR alpha 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
PPAR alpha antibody detects PPAR alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PPAR alpha stained by PPAR alpha antibody (GTX101098) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PPAR alpha antibody detects PPAR alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PPAR alpha stained by PPAR alpha antibody (GTX101098) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.
PPAR alpha antibody immunoprecipitates PPAR alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 μg of PPAR alpha antibody (GTX101098) 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody (GTX101098) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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文献和实验Chen HJ et al., Evid Based Complement Alternat Med 2021 (PMID:34257694)
Igarashi Y et al., Biomed Pharmacother 2021 (PMID:34022607)
Dai J et al., Elife 2021 (PMID:33496266)
Wang CY et al., Int J Med Sci 2021 (PMID:33390794)
Banerjee A et al., Sci Rep 2020 (PMID:32963259)
Guo S et al., Brain Res 2019 (PMID:31356784)
Wang H et al., Am J Physiol Endocrinol Metab 2019 (PMID:30457912)
Kubo H et al., Lipids Health Dis 2017 (PMID:28578672)
Orkhon B et al., Mol Med Rep 2018 (PMID:30272268)
Song L et al., Sci Rep 2017 (PMID:28169366)
Chung IH et al., Oncotarget 2016 (PMID:26968954)
Ye JH et al., Sci Rep 2016 (PMID:27612024)
Xu P et al., Nutr Metab (Lond) 2015 (PMID:26690553)
Hsu CY et al., Biogerontology 2014 (PMID:24973265)
Lai YS et al., J Agric Food Chem 2014 (PMID:24857364)
Hsu CY et al., J Gerontol A Biol Sci Med Sci 2013 (PMID:24149426)
Ho FM et al., J Ethnopharmacol 2012 (PMID:22543166)
Takuro Toda et al., Commun Biol 2022 (PMID:35552531)
Ling T et al., J Med Chem 2019 (PMID:31294974)
Lin CH et al., Nutrients 2021 (PMID:34836239)
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