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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP alpha. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Sheep
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX100674
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P, IP, ChIP assay
- 浓度:
0.59 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
C/EBP alpha
- 抗体英文名:
C/EBP alpha antibody [N1], N-term
- 抗体名:
C/EBP alpha 抗体 [N1], N-term
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (GTX100674) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (GTX100674) diluted at 1:500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Cross-linked ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or anti-C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献和实验Chen CC et al., Transl Res 2019 (PMID:31121128)
de Kerckhove M et al., EMBO Mol Med 2018 (PMID:30171089)
Wu JS et al., Mol Neurobiol 2017 (PMID:28942553)
Chen WC et al., PLoS One 2015 (PMID:26231035)
Chen YF et al., PLoS One 2015 (PMID:26121644)
Yang H et al., Anim Biosci 2022 (PMID:34530507)
Liu H et al., Diabetes Metab Syndr Obes 2020 (PMID:32943896)
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