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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX2. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX100656
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P
- 浓度:
0.42 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
COX2
- 抗体英文名:
COX2 antibody [C3], C-term
- 抗体名:
COX2 抗体 [C3], C-term
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term (GTX100656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term (GTX100656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term (GTX100656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using COX2(GTX100656) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Zhao Z et al., J Neuroimmune Pharmacol 2019 (PMID:31321663)
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补体成分 C3(C3a/C3b) 补体成分:C3 血清浓度(μg/ml):550-1200 分子量(kDa):195 亚单位(链)及分子量(kDa):α∶110;β∶85 激活产物:C3a;C3b 生物学活性:C3a 过敏毒素、趋化作用等。 C3b 组成CP、AP中的C3、C5转化
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠补体 C3(C3)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 C3 单抗包被于酶标板上,标准品和样品中的 C3 与单抗结合,加入生物素化的抗大鼠 C3 ,形成免疫复合物连接在板上,辣根
人补体C3 (C3)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 C3 单抗包被于酶标板上,标准品和样品中的 C3与单抗结合,加入生物素化的抗人 C3 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测
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