- ￥1700 - 4000
- GeneTex
- 美国
- GTX106197
- 2025年07月16日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human WNK1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX106197
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1.18 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
WNK1
- 抗体英文名：
WNK1 antibody
- 抗体名：
WNK1 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody (GTX106197) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody (GTX106197) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
WNK1 antibody detects WNK1 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
WNK1 antibody (GTX106197) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
WNK1 antibody detects WNK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
WNK1 stained by WNK1 antibody (GTX106197) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using WNK1(GTX106197) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunofluorescence analysis of methanol-fixed HeLa, using WNK1(GTX106197) antibody at 1:200 dilution.
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文献和实验Liu Y et al., J Am Soc Nephrol 2015 (PMID:25145935)
Ho YJ et al., In Vivo 2020 (PMID:32871793)
Ding L et al., JCI Insight 2017 (PMID:29202451)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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