- ￥3500
- GeneTex
- 美国
- GTX12412
- 2025年07月12日
- WB, ICC/IF, FACS, IP, ChIP assay, Gel supershift assays
- Mouse
- Human, Mouse, Rat, Rabbit, Bovine
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant PPAR alpha protein.
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Rabbit, Bovine
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX12412
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, FACS, IP, ChIP assay, Gel supershift assays
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
PPAR alpha
- 抗体英文名：
PPAR alpha antibody [3B6/PPAR]
- 抗体名：
PPAR alpha 抗体 [3B6/PPAR]
- 规格：
50 μg
FACS analysis of Hela cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% PFA
WB analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX12412 PPAR alpha antibody [3B6/PPAR].
Dilution : 1:1000 (lane 1 and 2) & 1:500 (lane 3)
FACS analysis of 3T3 cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% PFA
FACS analysis of Jurkat cells(1-5x10⁶ cells/ml) using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% PFA
ICC/IF analysis of HCT116 cells using GTX12412 PPAR alpha antibody [3B6/PPAR]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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文献和实验PPAR-Alpha Cloning, Expression, and Characterization
Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays
Combined Biophysical and Cell-Based Approaches for the Assessment of Ligand Binding to PPAR
and quantification of ligand binding to PPARγ. This method is specific for PPARγ while ANS displays negligible interaction with other nuclear receptors such as PPARα and retinoid X receptor α (RXRα). The ANS competition assay
Estimation of the PPAR Agonism of Fibrates by a Combined MM-Docking Approach
Fibrates are peroxisome proliferator-activated alpha receptor (PPARα) activators derived from fibric acid and are the most clinically used therapeutics in the treatment of hypertriglyceridemia. Recently, we reported a computational approach
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