- ￥1700 - 4000
- GeneTex
- 美国
- GTX121942
- 2025年07月15日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat, Dog
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human MEK1 + MEK2. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Dog
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX121942
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
MEK1 + MEK2
- 抗体英文名：
MEK1 + MEK2 antibody
- 抗体名：
MEK1 + MEK2 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody (GTX121942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 + MEK2 antibody (GTX121942) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
MEK1 + MEK2 antibody detects MEK1 + MEK2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% PFA at RT for 15 min.
Green: MEK1 + MEK2 stained by MEK1 + MEK2 antibody (GTX121942) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX121942 diluted at 1:2000
MEK1+2 antibody detects MEK1+2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% PFA at RT for 15 min.
Green: MEK1+2 protein stained by MEK1+2 antibody (GTX121942) diluted at 1:500.
Blue: Hoechst 33342 staining.
MEK1+2 antibody detects MEK1+2 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
MEK1+2 antibody (GTX121942) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Liu SM et al., Oncotarget 2014 (PMID:25275294)
Lin CY et al., Cell Transplant 2014 (PMID:25333338)
Hsieh YH et al., Nutrients 2018 (PMID:30200495)
is a highly selective inhibitor of MEK1 and MEK2 with IC50 values of 4 ?M and 50 ?M respectively. PD98059 does not inhibit activation of other highly related dual-specificity protein kinases or the activity of over 18 other Ser/Thr protein kinases
Mutational and Functional Analysis in Human Ras/MAP Kinase Genetic Syndromes
) syndrome, is caused by germline mutations in BRAF , MAP2K1 (MEK1 ) and MAP2K2 (MEK2 ), and possibly KRAS genes. Here, we describe the laboratory protocols and methods that we used to identify mutations in BRAF and MEK1/2 genes as causative for CFC syndrome
蛋白Ras的GDP解离而结合GTP，从而激活Ras;激活的Ras进一步与丝/苏氨酸蛋白激酶Raf-1的氨基端结合，通过未知机制激活Raf-1;Raf-1可磷酸化MEK1/MEK2(MAP kinase/ERK kinase)上的二个调节性丝氨酸，从而激活MEKs;MEKs为双特异性激酶，可以使丝/苏氨酸和酪氨酸发生磷酸化，最终高度选择性地激活ERK1和ERK2(即p44MAPK和p42MAPK)。ERKs为脯氨酸导向的丝/苏氨酸激酶，可以磷酸化与脯氨酸相邻的丝/苏氨酸。在丝裂原刺激后，ERKs接受上游
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