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- 文献和实验
- 技术资料
- 免疫原:
synthetic peptide corresponding to the C-terminal region of human histone deacetylase 2 (amino acid residues 471-488 with N-terminal added cysteine) conjugated to maleimide-activated KLH.
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Bovine, Dog, Chicken
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX12169
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IP, ELISA, IHC
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
HDAC2
- 抗体英文名:
HDAC2 antibody [HDAC2-62]
- 抗体名:
HDAC2 抗体 [HDAC2-62]
- 规格:
100 μl
IP analysis of HeLa cell lysate using GTX12169 HDAC2 antibody[HDAC2-62].
Lane 1 : IP from HeLa cell lysate using 5 μg antibody
Lane 2 : IP from HeLa cell lysate using 2.5 μg antibody
Lane 3 : IP from HeLa cell lysate using 5 μg non-relevant antibody
ICC/IF analysis of HeLa cells using GTX12169 HDAC2 antibody [HDAC2-62] at 10 μg/mL. Cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone.
WB analysis of (1)HEK-293T (2) HeLa (3) JURKAT (4) K562 (5) Neuro-2a (6) NIH-3T3 cell lysate using GTX12169 HDAC2 antibody [HDAC2-62] at 0.5 μg/mL.
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文献和实验Li Z et al., Oncotarget 2017 (PMID:28978036)
Gemoll T et al., Cell Mol Life Sci 2011 (PMID:21290163)
) 特异 HDAC class I/II 抑制剂,对 HDAC 的 IC 50 值为 1.8 nM; Phase 1 abs817901 128517-07-7 Romidepsin Pan-HDAC 抑制剂,抑制 HDAC1, HDAC2, HDAC4 和 HDAC6,IC 50 值分别为 36 nM, 47 nM, 510 nM 和 1.4 μM abs811955 99-66-1 Valproic Acid Pan-HDAC 抑制剂,抑制 HDAC1 的活性,(IC 50 =400 μM
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Antibody Precipitation with Protein A or G
Antibody Precipitation with Protein A or G Author: James Turmo Source: Contributed by James Turmo Abstract: Using Immobilized Protein A or Protein G
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