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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX115378
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP
- 浓度:
1.57 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
NDP52
- 抗体英文名:
NDP52 antibody
- 抗体名:
NDP52 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
NDP52 antibody detects NDP52 protein at autophagosome by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: NDP52 stained by NDP52 antibody (GTX115378) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.
Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody (GTX115378).
Western blot analysis was performed using NDP52 antibody (GTX115378).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NDP52(GTX115378) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX115378 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Sabrina Robichaud et al., Autophagy 2021 (PMID:33590792)
Shiho Fukushima et al., Front Cell Infect Microbiol 2022 (PMID:35310852)
Po-Yuan Ke et al., Cells 2022 (PMID:35406696)
Hall BS et al., Autophagy 2021 (PMID:34424124)
Hung CM et al., Sci Adv 2021 (PMID:33827825)
Ogawa M et al., Commun Biol 2020 (PMID:31932716)
Liao CC et al., Autophagy 2018 (PMID:30081720)
Wang X et al., Biomed Pharmacother 2020 (PMID:32361163)
Zachari M et al., Dev Cell 2019 (PMID:31353311)
Fili N et al., Nat Commun 2017 (PMID:29187741)
Chen CH et al., Clin Cancer Res 2017 (PMID:29222162)
Kimura T et al., Acta Neuropathol Commun 2017 (PMID:28874186)
CD52 分子 常用单克隆抗体或代号: YTH66.9;(Campath- 1) 主要表达细胞: Leu,Eo [NL] 分子质量(kDa)和结构: gp25- 29 功 能: 补体介导溶解作用的靶分子 Aka Campath-1 CD52 antibodies are lytic for target cells, both with human complement and via antibody dependent cellular
2019 博士考哪家?且看 2018 年 52 高校复试分数大汇总
2018 年的博士研究生复试已基本结束,有人欢喜,喜的是即将踏上梦想的殿堂;有人忧愁,愁的是复试被刷;更让人忧愁的是,考博复试的分数线,都没过。小编也专门整理了 52 所医学相关院校的博士复试分数线,对今年有一定的回顾作用。更重要的是,对下一轮的博士报考,具有很好的参考价值。复旦大学上海交通大学四川大学华中科技大学解放军总医院空军军医大学 海军军医大学首都医科大学中南大学南方医科大学山东大学 同济大学医学院 中国中医科学院郑州大学重庆医科大学天津中医药大学湖北中医药大学英语整理完今年的考博
关于DEAE52纤维素的处理只有简单的几步,如下: 1.先将干粉的纤维素浸泡在蒸馏水中,一段时间大约3小时左右,我偏爱这个时间,去除杂质,最好抽干一下; 2.再用0.5mol/l的HCl溶液浸泡2小时,用去离子水洗净至PH中性,并抽干; 3.将抽干的纤维素再浸泡在0.5mol/l的NaOH溶液中2小时,用去离子水洗至中性,抽干。即可用了对于用过的纤维素,可以重复利用多次,但需要经过再生
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