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NDP52 antibody

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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • 2025年07月14日
  • WB, ICC/IF, IHC-P, IP
  • Rabbit
  • Human, Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX115378

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, ICC/IF, IHC-P, IP

    • 浓度

      1.57 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      NDP52

    • 抗体英文名

      NDP52 antibody

    • 抗体名

      NDP52 抗体

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    NDP52 antibody detects NDP52 protein at autophagosome  by immunofluorescent analysis.
    Sample: Mock and treated HeLa cells were fixed in 4% PFA at RT for 15 min.
    Green: NDP52 stained by NDP52 antibody (GTX115378) diluted at 1:1000.
    Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
    Blue: Fluoroshield with DAPI (GTX30920).
    Scale bar= 10 μm.

    产品细节图片2

    Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody (GTX115378).
    Western blot analysis was performed using NDP52 antibody (GTX115378).
    EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

    产品细节图片3

    Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片4

    Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NDP52(GTX115378) antibody at 1:100 dilution.
    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

    产品细节图片5

    NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
    The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
    The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片6

    Sample (30 μg of whole cell lysate)
    A: Raji
    10% SDS PAGE
    GTX115378 diluted at 1:1000
    The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片7

    Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片8

    NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
    The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
    The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Sabrina Robichaud et al., Autophagy 2021 (PMID:33590792)
    Shiho Fukushima et al., Front Cell Infect Microbiol 2022 (PMID:35310852)
    Po-Yuan Ke et al., Cells 2022 (PMID:35406696)
    Hall BS et al., Autophagy 2021 (PMID:34424124)
    Hung CM et al., Sci Adv 2021 (PMID:33827825)
    Ogawa M et al., Commun Biol 2020 (PMID:31932716)
    Liao CC et al., Autophagy 2018 (PMID:30081720)
    Wang X et al., Biomed Pharmacother 2020 (PMID:32361163)
    Zachari M et al., Dev Cell 2019 (PMID:31353311)
    Fili N et al., Nat Commun 2017 (PMID:29187741)
    Chen CH et al., Clin Cancer Res 2017 (PMID:29222162)
    Kimura T et al., Acta Neuropathol Commun 2017 (PMID:28874186)
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    文献支持
    NDP52 antibody
    ¥1700 - 4000