- ￥1700 - 4000
- GeneTex
- 美国
- GTX115351
- 2025年07月12日
- WB, ICC/IF, IHC-P
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human LRP130. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX115351
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
LRP130
- 抗体英文名：
LRP130 antibody [N2N3]
- 抗体名：
LRP130 抗体 [N2N3]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N2N3] (GTX115351) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using LRP130(GTX115351) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
LRP130 antibody [N2N3] detects LRP130 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% PFA/culture medium at 37ºC for 30 min.
Green: LRP130 protein stained by LRP130 antibody [N2N3] (GTX115351) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
5% SDS PAGE
GTX115351 diluted at 1:1000
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文献和实验Antibody Staining of Ovaries 1. Dissect ovaries in EBR 2. Transfer ovaries into an Eppendorf tube and add 100 µl devitellinizing buffer and 600-µl heptane 3. Agitate gently for 10 minutes
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
： 对于FcR高表达，或具有Fc高亲和性的细胞，例如髓系细胞系等，建议实验中添加FcR阻断剂，用于减少非特异反应；此外，对于检测或分析表达水平较低的表面抗原物时，也建议进行Fc阻断，以提高检测特异性。或处理复杂样本或复杂panel时，为了避免可能造成的非特异反应，也可尝试进行FcR阻断，以获得准确实验结果。 美天旎生物技术公司FcR阻断剂（130-059-901和130-092-575）开学大促，为您的实验增加一份准确度。 [1] Chiu ML, Goulet DR, Teplyakov
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