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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the N-terminus region of human Liprin alpha 1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX115098
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-Fr, IP, IHC
- 浓度:
0.98 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Liprin alpha 1
- 抗体英文名:
Liprin alpha 1 antibody [N1N2], N-term
- 抗体名:
Liprin alpha 1 抗体 [N1N2], N-term
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Liprin alpha 1 antibody [N1N2], N-term detects PPFIA1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
5 % SDS-PAGE
Liprin alpha 1 antibody [N1N2], N-term (GTX115098) dilution: 1:1000
Liprin alpha 1 antibody [N1N2], N-term detects Liprin alpha 1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% PFA at RT for 15 min.
Green: Liprin alpha 1 stained by Liprin alpha 1 antibody [N1N2], N-term (GTX115098) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Sample (30 ug of whole cell lysate)
A: JurKat
5% SDS PAGE
GTX115098 diluted at 1:10000
Immunoprecipitation of Liprin alpha 1 protein from A431 whole cell extracts using 5 μg of Liprin alpha 1 antibody [N1N2], N-term (GTX115098).
Western blot analysis was performed using Liprin alpha 1 antibody [N1N2], N-term (GTX115098).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Liprin alpha 1 antibody detects Liprin alpha 1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Liprin alpha 1 antibody (GTX115098) diluted by 1:1000.
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文献和实验Bernadzki KM et al., Sci Rep 2017 (PMID:28831123)
Gingras J et al., J Cell Sci 2016 (PMID:26769899)
domesticus x Mus spretus] F1 DNA). In the analysis of the antibody bound (B) and unbound (U) fractions (right panel), the bands representing the maternal and paternal alleles are indicated.MATERIALS Reagents100-bp DNA step ladder (for duplex PCR
hydroxylaseAssociation of a 14-3-3 protein with CMP-NeuAc:GM1 alpha 2,3-sialyltransferaseInteraction of 14-3-3 with signaling proteins is mediated by phosphoserineActivation-modulated association of 14-3-3 proteins with Cbl14-3-3 binding sequence in cytoplasmic domain
Antigen Design Sera Purification Tech Sheet
, the N-terminus is also frequently exposed and on the surface of the protein making it an ideal candidate for antibody generation. If a protein sequence is derived from the cDNA sequence, the leader sequence should not be included in the sequence selected
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