- ￥1700 - 4000
- GeneTex
- 美国
- GTX114650
- 2025年07月16日
- WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay, IHC
- Rabbit
- Human, Mouse, Rat, Chimpanzee
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the C-terminus region of human Brn2. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Chimpanzee
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX114650
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay, IHC
- 浓度：
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Brn2
- 抗体英文名：
Brn2 antibody
- 抗体名：
Brn2 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Brn2 antibody (GTX114650) The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.
Brn2 antibody immunoprecipitates Brn2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Brn2 protein by 2 μg of Brn2 antibody (GTX114650) 10% SDS-PAGE The immunoprecipitated Brn2 protein was detected by Brn2 antibody (GTX114650) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Brn2 antibody detects Brn2 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Brn2 antibody (GTX114650) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Brn2(GTX114650) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% PFA at RT for 15 min.
Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.
Brn2 antibody detects Brn2 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Brn2 antibody (GTX114650) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Brn2 antibody (GTX114650) diluted at 1:1000.
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文献和实验Fujino K et al., Am J Pathol 2015 (PMID:26482608)
Shimada M et al., EMBO J 2015 (PMID:26290337)
Tan C et al., Clin Med Insights Oncol 2014 (PMID:24653654)
Lee Y et al., Nat Neurosci 2012 (PMID:22522401)
Cheng-Yi Yang et al., Neurotherapeutics 2021 (PMID:34132974)
Zachary A Schaaf et al., Mol Autism 2022 (PMID:35733184)
M?rio A F Soares et al., Genes Dev 2021 (PMID:34168041)
Wang J et al., Cell Rep 2020 (PMID:33326788)
Seiya Yamada et al., iScience 2020 (PMID:32344379)
Kitajima R et al., Stem Cell Res 2020 (PMID:32151953)
Yoon SJ et al., Nat Methods 2019 (PMID:30573846)
Enriquez-Rios V et al., J Neurosci 2017 (PMID:28123024)
Wong WT et al., Elife 2017 (PMID:28323620)
Enriquez-Rios V et al., JNeurosci 2016 (Epub)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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