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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human Calcium binding protein p22. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX113936
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Calcium binding protein p22
- 抗体英文名:
Calcium binding protein p22 antibody
- 抗体名:
Calcium binding protein p22 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Calcium binding protein P22 antibody detects CHP protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
12% SDS-PAGE
Calcium binding protein P22 antibody (GTX113936) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Calcium binding protein P22(GTX113936) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Calcium binding protein P22 antibody detects CHP protein by western blot analysis.
A. 50 μg rat liver lysate/extract
12% SDS-PAGE
Calcium binding protein P22 antibody (GTX113936) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Calcium binding protein P22 antibody detects Calcium binding protein P22 protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Calcium binding protein P22 antibody (GTX113936) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 μg of whole cell lysate)
A: HeLa
B: HepG2 (GTX27900)
12% SDS PAGE
GTX113936 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Calcium binding protein P22 antibody detects Calcium binding protein P22 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% PFA at RT for 15 min.
Green: Calcium binding protein P22 protein stained by Calcium binding protein P22 antibody (GTX113936) diluted at 1:500.
Blue: Hoechst 33342 staining.
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文献和实验Wang M et al., Cell Death Differ 2019 (PMID:31527798)
Zhu XG et al., Mol Cell 2019 (PMID:30846317)
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986
广义的是指与钙离子进行特异结合的蛋白质之总称。与活体内钙的多种功能相应地有多种功能的蛋白质,可分别为以Ca 2 为活化因子的酶(血液凝固因子、各种蛋白酶、磷脂酶 A 2 和α - 淀粉酶等分解酶、膜的 Ca 2 运输 ATPase 、磷酸化酶 b 激酶)及无酶活性的两类,后者多称为狭义的钙结合蛋白,其中又分为:( 1 )与酶或细胞的机能调控有关的;( 2 )通过与 Ca 2 结合使游离的离子浓度降低,具有贮藏 Ca 的功能的。但也有功能未被测定的。肌宁蛋白 C 及调钙蛋白
Characterization of Calcium Channel Binding
calcium channels are expressed in a variety of tissues including heart, muscles and brain. Saturation binding of a radioligand to the calcium channel is commonly used to characterize the expression level of the channel protein. Compound competition binding
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