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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human HADHA. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX113727
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.37 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
HADHA
- 抗体英文名:
HADHA antibody [N2C1], Internal
- 抗体名:
HADHA 抗体 [N2C1], Internal
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HADHA antibody [N2C1], Internal (GTX113727) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HADHA antibody [N2C1], Internal detects HADHA protein by Western blot analysis.
A.30 μg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
HADHA antibody [N2C1], Internal (GTX113727) dilution: 1:500
Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
7.5% SDS PAGE
GTX113727 diluted at 1:1000
HADHA antibody [N2C1], Internal detects HADHA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: HADHA protein stained by HADHA antibody [N2C1], Internal (GTX113727) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HADHA(GTX113727) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX113727 diluted at 1:1000
HADHA antibody [N2C1], Internal detects HADHA protein at Mitochondia by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min.
Green: HADHA protein stained by HADHA antibody [N2C1], Internal (GTX113727) diluted at 1:500.
Blue: Hoechst 33343 staining.
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文献和实验Dang Y et al., Biomed Pharmacother 2020 (PMID:32325349)
Harel M et al., Cell 2019 (PMID:31495571)
Lee J et al., Cell Rep 2016 (PMID:27320917)
Lee J et al., Cell Rep 2015 (PMID:25578732)
Rodriguez S et al., BMC Biochem 2014 (PMID:25152047)
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