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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Caspase 14. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX111888
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Caspase 14
- 抗体英文名:
Caspase 14 antibody
- 抗体名:
Caspase 14 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Caspase 14 antibody detects Caspase 14 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 14 antibody (GTX111888) at a dilution of 1:1000.
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX111888 diluted at 1:1000
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文献和实验Dang N et al., Iran J Basic Med Sci 2016 (PMID:27096061)
或 C6 cells 作为 cleaved caspase-3 的阳性参照。更多靶点的阳性对照,请登陆CST网站——研究——靶标的阳性对照处理。图二 蛋白质印迹分析(图源自CST Caspase-3 Antibody #9662 抗体说明书)为什么所有的实验都要设置对照?无论是初高中刚接触生物学实验设计还是上研究生后发表 SCI 文章,老师们以及 reviewer 都会敲着「黑板」强调强调再强调,每一个实验都必须有对照!因为设置适当的阴性、阳性甚至空白对照对于实验结果的准确、有效阐释至关重要!阅历丰富
【求助】请问western blot做凋亡检测时,识别caspase-3酶原和激活的caspase-3的抗体是相同的吗?
-caspase-3(#9664)和原形态的caspase-3(#9662),但是需要说明的是,原形态的caspase-3抗体也可以同时检测剪切态的caspase-3,所以建议购买原形态的caspase-3就行了。 (如下图所示) 另外,PARP也是购买全长的就行了,(PARP Antibody #9542)。 lsdcfhyj 谢谢两位的回答 本文由丁香园论坛提供,想了解更多有用的、有意思的前沿资讯以及酷炫的实验
of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins. A polyclonal antibody was raised against purified ST-IV. A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
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