- ￥1700 - 4000
- GeneTex
- 美国
- GTX111809
- 2025年07月13日
- WB, IHC-P
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human Calpain 2. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX111809
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
0.47 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Calpain 2
- 抗体英文名：
Calpain 2 antibody [N2C1-2], Internal
- 抗体名：
Calpain 2 抗体 [N2C1-2], Internal
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111809 diluted at 1:1000
Calpain 2 antibody [N2C1-2], Internal detects Calpain 2 protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Calpain 2 antibody [N2C1-2], Internal (GTX111809) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Calpain 2(GTX111809) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 2 antibody [N2C1-2], Internal (GTX111809) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Yang HH et al., Int J Mol Sci 2022 (PMID:35163312)
RUVKUN Antibody Staining Protocol revised 10/29/90
amts!, don't use up sample) with 1% toluidine blue. Rinse several times in buffer B. Compare two samples at 50X under dissecting scope. If animals are " open" , + RNAse animals will have very little staining of internal tissues. ANTIBODY INCUBATIONS
domesticus x Mus spretus] F1 DNA). In the analysis of the antibody bound (B) and unbound (U) fractions (right panel), the bands representing the maternal and paternal alleles are indicated.MATERIALS Reagents100-bp DNA step ladder (for duplex PCR
Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
and 50 mM dithiothreitol (DTT; Sigma) 2 mM N ‐ethylmaleimide (NEM; Sigma) 2.5% (w/v) 5‐sulfosalicylic acid (SSA; Sigma
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