- ￥1700 - 4000
- GeneTex
- 美国
- GTX111095
- 2025年07月15日
- WB, ICC/IF, IHC-P, IHC-Fr
- Rabbit
- Human, Mouse, Rat, Zebrafish, Cat, Dog
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human alpha 1 Catenin. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Zebrafish, Cat, Dog
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX111095
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr
- 浓度：
0.87 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
alpha 1 Catenin
- 抗体英文名：
alpha 1 Catenin antibody [N3C2], Internal
- 抗体名：
alpha 1 Catenin 抗体 [N3C2], Internal
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha 1 Catenin antibody (GTX111095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha 1 Catenin antibody (GTX111095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha 1 Catenin antibody [N3C2], Internal detects alpha 1 Catenin protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1 Catenin antibody [N3C2], Internal (GTX111095) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX111095 diluted at 1:10000
alpha 1 catenin antibody [N3c2], Internal detects alpha 1 Catenin protein at cytoplasm and cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: alpha 1 Catenin protein stained by alpha 1 catenin antibody [N3c2], Internal (GTX111095) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
alpha 1 Catenin antibody [N3C2], Internal detects alpha 1 Catenin protein at cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: alpha 1 Catenin stained by alpha 1 Catenin antibody [N3C2], Internal (GTX111095) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha 1 Catenin antibody [N3C2], Internal (GTX111095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha 1 Catenin antibody [N3C2], Internal detects alpha 1 Catenin protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha 1 Catenin antibody [N3C2], Internal (GTX111095) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 ug of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX111095 diluted at 1:10000
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文献和实验Li X et al., Mol Cell 2018 (PMID:30392930)
Stewart S et al., Cell Rep 2014 (PMID:24485659)
，后来发现与我买的beta-catenin一抗（中杉金桥分装的santa cruz的产品）说明书上的结果一致。请问大家做beta-catenin免疫荧光用的是哪里的抗体啊，或者是我的方法有问题吗？我也用0.5%TritonX-100打孔了，仍不行。我在这里先谢谢各位了！ zhao22840718 还有一种方法不知有没有人试过，就是把beta-catenin构建到pEGFP-N3类载体中，表达融合蛋白，把载体转入细胞，然后加入你的药物。荧光显微镜下观察进核情况
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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