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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human DDX3. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX110614
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
1.87 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
DDX3
- 抗体英文名:
DDX3 antibody [N3C2], Internal
- 抗体名:
DDX3 抗体 [N3C2], Internal
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% PFA at RT for 15 min.
Green: DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.
Immunofluorescence analysis of PFA-fixed Human ESC, using DDX3(GTX110614) antibody at 1:200 dilution.
U87-MG whole cell and cytoplasm extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000.
Sample (20 ug of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX110614 diluted at 1:2000
DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Sundararaman B et al., Mol Cell 2016 (PMID:26990993)
Sarah C Atkinson et al., Int J Mol Sci 2021 (PMID:34445801)
Deschepper CF et al., Sci Rep 2020 (PMID:32913328)
Tsai WC et al., Appl Immunohistochem Mol Morphol 2018 (PMID:29621097)
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