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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 8. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX110432
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
alpha Tubulin 8
- 抗体英文名:
alpha Tubulin 8 antibody [N1C1-2]
- 抗体名:
alpha Tubulin 8 抗体 [N1C1-2]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin 8(GTX110432) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX110432 diluted at 1:5000
Immunofluorescence analysis of PFA-fixed A431, using alpha Tubulin 8(GTX110432) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hela
10% SDS PAGE
GTX110432 diluted at 1:1000
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文献和实验Sharma S et al., Genome Biol 2019 (PMID:30791937)
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
immunofluorescence. Block in AbDil for 10'. Anti-tubulin for 20' - 30' (We use DM1alpha) Wash 4x TBST (TBST = TBS + 0.1% Triton X-100) Secondary for 20' - 30' Wash 4X TBST. Wash once with TBST + 1 ug/ml Hoechst
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
'. Anti-tubulin for 20' - 30' (We use DM1alpha) Wash 4x TBST (TBST = TBS + 0.1% Triton X-100) Secondary for 20' - 30' Wash 4X TBST. Wash once with TBST + 1 ug/ml Hoechst. A rapid rinse will be sufficient. Drain, mount and seal. COMMENT
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
'. Anti-tubulin for 20' - 30' (We use DM1alpha) Wash 4x TBST (TBST = TBS + 0.1% Triton X-100) Secondary for 20' - 30' Wash 4X TBST. Wash once with TBST + 1 ug/ml Hoechst. A rapid rinse will be sufficient
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