Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse, Rat
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX109877
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IHC-P
浓度:
1.49 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
Fumarate hydratase
抗体英文名:
Fumarate hydratase antibody
抗体名:
Fumarate hydratase 抗体
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody (GTX109877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Fumarate hydratase antibody detects Fumarate hydratase protein by western blot analysis. A.50 μg rat liver lysate/extract 10% SDS-PAGE Fumarate hydratase antibody (GTX109877) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarate hydratase stained by Fumarate hydratase antibody (GTX109877) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
Fumarate hydratase antibody detects FH protein by western blot analysis. A. 50 μg mouse liver lysate/extract 7.5% SDS-PAGE Fumarate hydratase antibody (GTX109877) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase (GTX109877) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody (GTX109877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Application Reference
Yi-Wen Lo et al., Journal of Cellular and Molecular Medicine 2015 (PMID:25639359)
Miettinen M et al., Am J Surg Pathol 2016 (PMID:27454940)