Carbohydrate Labeling and Anal

The PA 800 plus Carbohydrate Labeling and Analysis Chemistry contains the reagents and separation capillary required to label, separate and quantify oligosaccharides and monosaccharides released from glycoproteins. Following release (enzymatic or chemical) from proteins, glycans are labeled with a fluorophore, APTS, at the reducing terminus by reductive amination. The stoichiometry of labeling is such that only one APTS molecule is attached to each molecule of oligosaccharide. These highly charged and fluorescent oligosaccharides are easily resolved in an electric field and detected using laser-induced fluorescence. Includes: • Carbohydrate Separation Buffer, 56 mL (Part Number 477623) • N-CHO Coated Capillary, qty 2 (Part Number 477601) • Labeling Reagents –Labeling Dye (APTS), 4 x 5 mg –Labeling Dye Solvent, 1 mL (Part Number 501309) –Glucose Ladder Standard, 50 mg –Quantitation/Mobility Marker (Maltose), 0.18 mg • APTS-M (APTS extra purified for monosaccharide labeling) • Carbohydrate Labeling and Analysis Guide