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- NEB
- 美国
- #M0545L
- 2025年10月06日
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- 详细信息
- 技术资料
- 保存条件：
-20
- 保质期：
1年
- 英文名：
Exonuclease VIII, truncated
- 库存：
999
- 供应商：
上海淳麦
- 规格：
1000U/10000U
Exonuclease VIII, truncated, is a genetically engineered active domain of exonuclease VIII from E. coli. Exonuclease VIII, truncated is able to initiate nucleotide removal from the 5´ termini of linear double-stranded DNA in the 5´ to 3´ direction (1). The enzyme does not degrade supercoiled dsDNA and circular ssDNA.
Product Source
An E. coli strain that carries a plasmid with genetic engineering active domain of Exonuclease VIII.
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| NEBuffer 4 | -20 | 10X |
Description
Exonuclease VIII, truncated, is a genetically engineered active domain of exonuclease VIII from E. coli. Exonuclease VIII, truncated is able to initiate nucleotide removal from the 5´ termini of linear double-stranded DNA in the 5´ to 3´ direction (1). The enzyme does not degrade supercoiled dsDNA and circular ssDNA.
Product Source
An E. coli strain that carries a plasmid with genetic engineering active domain of Exonuclease VIII.
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| NEBuffer 4 | -20 | 10X |
Advantages and Features
Applications
• Degradation of linear dsDNA while maintaining double stranded circular DNA.
Properties and Usage
Unit Definition
One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide from double-stranded DNA in a total reaction volume of 50 µl in 30 minutes at 37°C in 1X NEBuffer 4 with 0.15 mM sonicated duplex [3H] DNA.
Reaction Conditions
1X NEBuffer 4
Incubate at 37°C
1X NEBuffer 4:
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
1 mM DTT
pH 7.9 @ 25°C
Storage Temperature
-20°C
Storage Conditions
50 mM Tris-HCl
100 mM NaCl
0.1 mM EDTA
1 mM DTT
0.1% Triton® X-100
50% Glycerol
pH 7.5 @ 25°C
Heat Inactivation
70°C for 15 min
Unit Assay Conditions
1X NEBuffer 4 with 0.15 mM with sonicated duplex 3H- DNA.
References
1. Chang, et al. (2001). J. Biol. Chem.. 46004–46010.
上海淳麦生物科技有限公司专注于感受态细胞、质粒、载体、农杆菌、酵母等生命科学领域的产品研发和技术服务支持。
上海淳麦生物科技有限公司在感受态细胞的研发方面，实力雄厚，拥有了克隆、表达、酵母和农杆菌4大类，共计百余种感受态细胞。在电击感受态方面，拥有了大肠杆菌和农杆菌电击感受态种类，极大提高了客户在转基因以及文库构建方面的成功率。
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