- ￥5300
- GeneTex
- 美国
- GTX19350
- 2025年07月14日
- WB, ICC/IF, IP, Neutralizing/Inhibition
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A431 trypsinized membranes.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX19350
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP, Neutralizing/Inhibition
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
P-Cadherin
- 抗体英文名：
P-Cadherin antibody [6A9]
- 抗体名：
P-Cadherin 抗体 [6A9]
- 规格：
100 μg
WB analysis of 25 ug of 293T (lane 1), A431 (lane 2) and mouse brain (lane 3) using GTX19350 P-Cadherin antibody [6A9].
Dilution : 1:500
ICC/IF analysis of BEAS-2B Cells using GTX19350 P-Cadherin antibody [6A9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
ICC/IF analysis of HeLa cells using GTX19350 P-Cadherin antibody [6A9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
ICC/IF analysis of A2058 Cells using GTX19350 P-Cadherin antibody [6A9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
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文献和实验Single Primer ("Semi-Random") P
cresol red 2. T/TS 10.5 µL EDB 1 µL Taq Polymerase (Promega) @ 5 u/µL 1 µL TaqStart antibody (CloneTech) as delivered 3. EDB 2.5 mg/ml bovine serum albumin in 10 mM Tris, pH 8.3 References 1. S.R.J.A.M. Hermann
. When Biotinylating an antibody, you can test the antibody by Flow Cytometry. 6. F/P Ratio: a) Dilute an aliquot of FITC-Ig complex with buffer so that the Absorbance at 280nm (A280) is less than 2.0 b) Determine and record A280 and A492. c) Calculate
, you can determine the FITC to Protein ratio. When Biotinylating an antibody, you can test the antibody by Flow Cytometry. 6. F/P Ratio: a) Dilute an aliquot of FITC-Ig complex with buffer so that the Absorbance at 280nm (A280) is less than 2.0
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