- ￥5300
- GeneTex
- 美国
- GTX22724
- 2025年07月12日
- WB, ICC/IF, FACS, IP, ELISA, IHC
- Mouse
- Human, Mouse, Rat, Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide corresponding to residues M(281) H R Q E T(p) V D C L K K F N(294) of rat CaM Kinase II protein.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Rabbit
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Rat
- 目录编号：
GTX22724
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, FACS, IP, ELISA, IHC
- 浓度：
1 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
CaMKII alpha (phospho Thr286)
- 抗体英文名：
CaMKII alpha (phospho Thr286) antibody [22B1]
- 抗体名：
CaMKII alpha (phospho Thr286) 抗体 [22B1]
- 规格：
100 μl
ICC/IF analysis of U251 cells using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
ICC/IF analysis of Neuro-2a cells (left panel) or cells stimulated with Lamda-Phosphatase (Lambda-PPase) (right panel) using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
WB analysis of unphosphorylated and phosphorylated rat brain homogenate (left and right lanes respectively) using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1] or GTX22725CaMKII alpha antibody [6G9] .
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文献和实验Lee LC et al., Mol Brain 2021 (PMID:34607601)
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
9921 Phospho-PKC Antibody Sampler Kit 9375 Phospho-PKCalpha/beta II (Thr638/641) Antibody W IP 9374 Phospho-PKCdelta (Thr505) Antibody W IHC 9376 Phospho-PKCdelta/theta (Ser643/676) Antibody W IHC 9377 Phospho-PKCtheta (Thr538
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