- ￥5000
- GeneTex
- 美国
- GTX22746
- 2025年07月13日
- WB, ICC/IF, IHC-P, FACS, IP, Gel supershift assays, IHC
- Mouse
- Human, Mouse, Rat, Bovine, Fish, Primate, Avian
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide corresponding to the residues E(247) V G M M K G G I R K D R R G(261) of the ER alpha DNA binding domain.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Bovine, Fish, Primate, Avian
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX22746
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, IP, Gel supershift assays, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody detects both the steroid occupied and unoccupied forms of the estrogen receptor (ER) alpha from human samples. It detects ER alpha and does not cross react with ER beta.
- 抗体英文名：
Estrogen Receptor alpha antibody [33]
- 抗体名：
Estrogen Receptor alpha 抗体 [33]
- 规格：
50 μg
WB analysis of full-length human ER alpha recombinant protein and ER beta protein using GTX22746 Estrogen Receptor alpha antibody [33] at 4 μg/ml (left panel) or GTX25786 Estrogen Receptor beta antibody at 1:2000 (right panel).
WB analysis of 25 ug of the indicated whole cell lysates using GTX22746 Estrogen Receptor alpha antibody [33].
Dilution : 4 μg/ml
ICC/IF analysis of T-47D cells using GTX22746 Estrogen Receptor alpha antibody [33].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 10 μg/ml overnight at 4ºC
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文献和实验Massinen S et al., Hum Mol Genet 2009 (PMID:19423554)
Kao TL et al., Theranostics 2018 (PMID:29774067)
Kao T.L. et al., Theranostics 2018;8(10)
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
Figure 13.6.3 Glucocorticoid receptor [F602S] LBD binding to the coactivator peptide PGC‐1 alpha (130‐154) (biotin‐DGTPPPQEAEEPSLLKKLLLAPANT‐CONH2) (A ) or the corepressor peptide NCoR (2251‐2275) (biotin
【翻译】Development trends for monoclonal antibody cancer therapeutics
; ImClone/Bristol-Myers Squibb), nimotuzumab (TheraCIM; YM BioSciences) and panitumumab (Vectibix; Amgen). These products inhibit binding of EGF and subsequent activation of the EGF receptor33, 34. The in vivo mechanism of action of trastuzumab
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