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- 文献和实验
- 技术资料
- 免疫原:
Purified bovine brain cytoplasmic dynein.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Bovine
- 目录编号:
GTX80684
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-Wm, FACS, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody detects two isoforms of the ~74 kDa polypetide of cytoplasmic dynein.
- 抗体英文名:
Dynein antibody [74.1]
- 抗体名:
Dynein 抗体 [74.1]
- 规格:
100 μg
ICC/IF analysis of C6 cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
FACS analysis of C6 cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% PFA
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
FACS analysis of U87-MG cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% PFA
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
ICC/IF analysis of HeLa cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
ICC/IF analysis of U-87 MG cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
FACS analysis of Hela cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% PFA
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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文献和实验Huang FI et al., Front Oncol 2019 (PMID:31024851)
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Coating of Platelets with Antibody in vitro
OUTLINE Antibody-coated platelets (opsnized) may be used in the subsequent thrombophagocytosis assay. PROTOCOL Resuspend 1.6x10^8 of CMFDA-labeled platelets in 200µl of BSGC-BSA (v/w 1%) either with a control Ab
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controls axonemal dynein activity, flagellar beat, and male fertility. Science(2021).https://doi.org/ 10.1126/science.abd49146. Naphak Modhiran et al. A broadly protective antibody that targets the flavivirus NS1 protein. Science(2021).https://doi.org/ 10
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