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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX80695
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IP, ChIP assay, Gel supershift assays
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
KAP1
- 抗体英文名:
KAP1 antibody [20C1]
- 抗体名:
KAP1 抗体 [20C1]
- 规格:
100 μg
ICC/IF analysis of gamma irradiated HeLa cells using GTX80695 KAP1 antibody [20C1].
ICC/IF analysis of HeLa cells using GTX80695 KAP1 antibody [20C1]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
WB analysis of nuclear enriched extracts (30 μg lysate) of HEK 293 treated with Hydrogen peroxide (100um Hydrogen peroxide for 10 min) (Lane 1), HEK 293 (Lane 2), A549 (Lane 3), HEL 92.1.7 (Lane 4), PC-3 (Lane 5), and Caco-2 (Lane 6) using GTX80695 KAP1 antibody [20C1].
Dilution : 1 μg/ml
WB analysis of gamma irradiated HeLa cells using GTX80695 KAP1 antibody [20C1].
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文献和实验Protocol for anti-HA antibody Western Blotting
room. Expose film for ~2 mins. Notes on the primary antibody: Source - Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in ―20c
Isolating primary B cells from human blood
of PBS/EDTA 3. carefully layer 35 ml of diluted cell suspension over 15ml Ficoll Paque (1.077 density) in a 50 ml conical tube and spin at 400g for 40min at 20C in a swinging bucket rotor without brake 4. aspirate upper layer, leaving
can be taken into 100% MeOH and stored at —20C at this point. 6. Rehydrate the embryos in a series of 50% MeOH (20 min) -> PBS (2x 15 min) -> freshly prepared ice-cold PBSMT (2 x 15 min, 1 x 1-2hour). 7. Incubate with primary antibody in PBMST at 4oC
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