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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antiserum was produced against synthesized phosphopeptide derived from human IKK-a/b around the phosphorylation site of serine 176 (Q-G-Sp-L-C).
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX79039
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
IKK alpha (phospho Ser176) + IKK beta (phospho Ser177)
- 抗体英文名:
IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) antibody
- 抗体名:
IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) 抗体
- 规格:
100 μg
IHC-P analysis of human breast carcinoma tissue using GTX79039 IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) antibody.
WB analysis of NIH-3T3 cells using GTX79039 IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) antibody. Dilution : 1:1000
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文献和实验下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
9921 Phospho-PKC Antibody Sampler Kit 9375 Phospho-PKCalpha/beta II (Thr638/641) Antibody W IP 9374 Phospho-PKCdelta (Thr505) Antibody W IHC 9376 Phospho-PKCdelta/theta (Ser643/676) Antibody W IHC 9377 Phospho-PKCtheta (Thr538
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