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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The immunogen used to generate this antibody corresponds to bovine Casein.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Bovine
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Bovine
- 目录编号:
GTX37769
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, ELISA
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Casein
- 抗体英文名:
Casein antibody
- 抗体名:
Casein 抗体
- 规格:
200 μl
WB analysis of lysates from (1) mouse brain and (2) non-fat dry milk lysates (45ug per lane) using Casein antibody.
An ELISA plate is coated with 50 μL of non-fat milk at 10 μg/mL. The coated protein is detected with anti-Casein antibody (GTX37769) at concentration ranged from 0.2 to 0.03125 ng/mL.
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文献和实验Kleinjan M et al., J Nutr 2021 (PMID:33768229)
Saenger T et al., J Pharm Biomed Anal 2014 (PMID:24747146)
Ho CH et al., J Dairy Res 2010 (PMID:20822569)
Lara Bosco et al., Insects 2020 (PMID:33291265)
Calpain Zymography with Casein or Fluorescein Isothiocyanate Casein
Methods for analyzing enzyme activity after electrophoresis in acrylamide gels have been described for a variety of proteins, including several proteases and kinases. The application of this approach to detect calpain activity by casein
一种含有微量糖的磷蛋白。是乳蛋白的主要成分。乳中酪蛋白含量(%):人为 0.9,马为 1.3,牛为 2.5(约相当于牛乳总蛋白的 80%)。在等电点 pH4.6附近不溶于水,通常利用这种性质,使脱脂牛乳在弱酸下,生成的沉淀于中性附近溶于水,再使之在酸性条件下沉淀,如此反复操作来制造纯品。此标准样品(酸酪蛋白)在电泳上仍是不一致的,可分为α -酪蛋白、κ -酪蛋白和β -酪蛋白。各种酪蛋白具有容易聚合的性质,单体分子量为 2— 3万。每一种都含磷。此外还知有γ -酪蛋白。酪蛋白含有所有的氨
Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose
either to immobilized substrates, like casein-Sepharose (4 ,5 ), or to the inhibitor calpastatin (6 ), is highly selective but has the inherent risk that autoproteolysis may alter the calpain while it is being purified. Inactivation of calpain through mutation
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