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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
anti-μ-stimulated human B lymphocytes
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性:
Monoclonal
- 标记物:
PerCP
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX80158
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
FACS
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
CD69
- 抗体英文名:
CD69 antibody [FN50] (PerCP)
- 抗体名:
CD69 抗体 [FN50] (PerCP)
- 规格:
100 test
Surface staining of human peripheral blood using anti-CD69 antibody (clone FN50) after overnight activation of T cells by anti-CD3 (clone MEM-57).
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文献和实验S?nchez-Villanueva Jos? Antonio et al., bioRxiv 2019 (Epub)
S?nchez-Villanueva JA et al., PLoS One 2019 (PMID:31841528)
-KLH 同型对照专为胞内检测设计,省去昂贵与繁琐的重组细胞因子竞争封闭步骤。3、 激活对照激活对照使用细胞表面表达 CD69 来评价激活与否。如果未达到 CD69 期望的水平,则激活步骤出了问题,某一试剂可能失活,过期,制备不当或溶剂污染,需制备新鲜刺激剂重新实验。a. 取部分血样加 PMA+I 但是不加 BFA( 抑分泌试剂如 BFA 会影响 CD69 表达,需去除以进行表面标志检测 ) 。b. 表面染色 CD69PE/CD3PerCP ,省略
Fluorochrome Absorption and Emission Spectra
., RED613™ , EDC, PerCP), BD Cy-Chrome™ is a superior fluorochrome for third color analysis because of its high emission intensity and broad spectrum. As with our R-PE conjugates, an average of one BD Cy-Chrome™ molecule is coupled per antibody or protein
Fluorochrome Absorption and Emission
., RED613™ , EDC, PerCP), BD Cy-Chrome™ is a superior fluorochrome for third color analysis because of its high emission intensity and broad spectrum. As with our R-PE conjugates, an average of one BD Cy-Chrome™ molecule is coupled per antibody or protein
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