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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full-length His-tagged recombinant murine FoxP3
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX80283
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, FACS
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody recognizes N-terminal region of FoxP3.
- 抗体英文名:
FOXP3 antibody [3G3]
- 抗体名:
FOXP3 抗体 [3G3]
- 规格:
100 μg
Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3).
Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3) (GTX80283)
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文献和实验Marc A Gavin et al., Proc Natl Acad Sci U S A . 2006 (PMID:16617117)
人Foxp3 ( Foxp3 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Foxp3 单抗包被于酶标板上,标准品和样品中的 Foxp3与单抗结合,加入生物素化的抗人 Foxp3 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,Foxp3 浓度与 OD 值
Intracellular Immunofluorescent Staining for Flow Cytometry
supernatant. [OPTIONAL] Block with 2% (2 µl) normal rat serum in 1X Permeabilization Buffer, in approximately 100 µl volume, at 4°C for 15 minutes. Without washing after blocking step, add 20 µl fluorochrome conjugated anti-human Foxp3 antibody
Intracellular Immunofluorescent Staining for Flow Cytometry
supernatant. [OPTIONAL] Block with 2% (2 µl) normal rat serum in 1X Permeabilization Buffer, in approximately 100 µl volume, at 4°C for 15 minutes. Without washing after blocking step, add 20 µl fluorochrome conjugated anti-human Foxp3 antibody
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