- ￥6200
- GeneTex
- 美国
- GTX43144
- 2025年07月15日
- FACS
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Murine LAG3 Ig fusion protein.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性：
Monoclonal
- 标记物：
PE
- 适应物种：
Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX43144
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
FACS
- 浓度：
0.2 mg/ml
- 靶点：
This antibody recognizes an epitope within the D2 domain of LAG3.
- 抗体英文名：
LAG3 antibody [C9B7W] (PE)
- 抗体名：
LAG3 抗体 [C9B7W] (PE)
- 规格：
100 μg
FACS analysis of C57Bl/6 splenocytes stimulated for 3 days with ConA stained APC anti-mouse CD3 antibody, followed by staining with GTX43144 LAG3 antibody [C9B7W] (PE).
Right panel : Co-stained with APC anti-mouse CD3 antibody and GTX43144
Left panel : Co-stained with APC anti-mouse CD3 antibody and PE rat IgG1 isotype control
Antibody amount : 0.5 μg
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文献和实验Scarlett UK et al., J Exp Med 2012 (PMID:22351930)
Meng Q et al., Invest Ophthalmol Vis Sci 2006 (PMID:17003438)
Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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