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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
anti-μ-stimulated human B lymphocytes
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性:
Monoclonal
- 标记物:
PE
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX80157
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
FACS
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
CD69
- 抗体英文名:
CD69 antibody [FN50] (PE)
- 抗体名:
CD69 抗体 [FN50] (PE)
- 规格:
100 test
Surface staining of human peripheral blood using anti-CD69 antibody (clone FN50) after overnight activation of T cells by anti-CD3 (clone MEM-57).
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文献和实验S?nchez-Villanueva Jos? Antonio et al., bioRxiv 2019 (Epub)
S?nchez-Villanueva JA et al., PLoS One 2019 (PMID:31841528)
Western Blotting with Horseradish Pe
7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED IN ALL STEPS
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
说明①细胞为小鼠脾细胞②抗体为PE标记的抗CD3抗体和FITC标记的抗CD45R抗体(2)结果以点状二维图表示。横坐标为B220分子,纵坐标为CD3分子。因此,左上为T细胞群,右下为B细胞群。(3)结果分析在脾脏中主要存在有两大类淋巴细胞群—T细胞和B细胞。其中B细胞占60%左右,T细胞占30%。3. 活化T细胞CD69分子的表达(1)简要说明①成熟T细胞来自正常小鼠脾脏,经纯化而获得,通常情况下T细胞处于静止状态,而在不同浓度的抗CD3抗体的刺激下T细胞活化。②抗体为FITC标记的抗CD69抗体
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